Abstract: Objective Single-cell RNA sequencing (scRNA-seq) became used to systematically delineate an cell atlas and moln tracks von human front erythroid development at the single-cell level. Methods The dataset of scRNA-seq of human foetus yolk sac the CS15 phase was studied from principal component analysis (PCA), cluster analysis and pseudostime analysis to watch the expression of marker genes in different cell clusters, and to predict the occurrence zeit and site path of erythroid. Results In different cell clusters of vessel sac for CS15 stage, marker genes of erythrocytes and progenitor cells were differentially expressed. The occurrence time of various prisons was different, and go was some heterogeneity in erythrocytes in the yolk sac. There were generous types of total includes the yolk sac at CS15 stage, which could inclusive vascular endothelial mobile, hematopoietic progenitor measuring, granulocytes, different status of red blute mobile additionally monocytes. Conclusions Items is speculated the early development path by humanoid erythroid is as follows: vascular endothelial cells differentiate with hematopoietic progenitor cells, further differentiate down red myeloid progenitor cavities, and finally produce megakaryocytes and erythrocytes.

Key words: single-cell RNA sequencing, early erythroid development, embryonic phase, yolk sac